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pguide ef1a gfp  (OriGene)


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    Structured Review

    OriGene pguide ef1a gfp
    Pguide Ef1a Gfp, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pguide+ef1a+gfp/pGuide/pmc13112475-62-5-6
    Average 90 stars, based on 4 article reviews
    pguide ef1a gfp - by Bioz Stars, 2026-09
    90/100 stars

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    Sequencing:

    Article Title: A homozygous nonsense variant in the oligosaccharyltransferase complex gene, RPN1 , causes a congenital disorder of glycosylation
    Article Snippet: .. HEK293 cells were co-transfected with pGuide-EF1a-GFP (OriGene GE100044 ) containing the guide RNA sequence 5′-AATGCAGAAGCTGGATGCAC-3′ targeting exon 10 of RPN1 and the base editor pcmv-BE4max (Addgene #112093). .. Transfected cells were recovered for 48 h, and subsequently GFP-positive cells were sorted into 96-well plates using a FACSAria IIu instrument (BD Biosciences) for isogenic clones.

    Article Title: A Homozygous Nonsense Variant in the Oligosaccharyltransferase Complex Gene, RPN1, Causes a Congenital Disorder of Glycosylation.
    Article Snippet: .. HEK293 cells were co-transfected with pGuide-EF1a-GFP (OriGene GE100044) containing the guide RNA sequence 5’-AATGCAGAAGCTGGATGCAC-3’ targeting exon 10 of RPN1 and the base-editor, pcmv-BE4max (Addgene #112093). .. Transfected cells recovered for 48 hours and subsequently, GFP-positive cells sorted into 96-well plates Jo urn al Pr e-p roo f 8 using a FACSAria IIu instrument (BD Biosciences) for isogenic clones.



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